Study Status: RECRUITING
Recruit Status: RECRUITING
Study Type: INTERVENTIONAL
Official Title: Environmental Factors and Microbiological Interactions in Structure of Dental Biofilm
Brief Summary: Orthodontic appliances can affect changes in the relationships of members of the oral microbiome.
Microbiome imbalance can result in oral infections and complicate treatment.
The focus is on the microbiological profile of dental biofilm and its control.
The effects of metals released by corrosion of alloys for oral use on the microbiome and the extent to which they modify the effectiveness of gingivitis therapy by mechanical and chemical control of biofilm will be investigated.
Early and late colonizers of the tooth surface will be analyzed.
The condition of gingiva will be assessed, and the results will show the specifics of biofilm and gingiva exposed and unexposed, and differences in the response of individual bacteria to therapy.
Molecular biology techniques will be used to quantify the total bacterial biomass (16s rRNA) and the proportion of specific bacterial species within the dental biofilm.